Fig 1: SOX2 induces IGF2/IGF1R signaling molecules in bladder cancer cells. (a) qPCR analysis to assess IGF2 expression in T24 cells transduced with the lentiviral vector encoding SOX2 cDNA (SOX2) or empty control vector (Ctrl). Results are the average of three replicates and expressed as the mean ± S.D. ***P < 0.001. (b) qPCR analysis to assess IGF2 expression in T24 versus 5637 cells (left) and in 5637 cells transduced with shSOX2 versus scrambled control (SC) vector (right). The #1 and #2 indicate the two distinct shRNAs that target different regions within SOX2. ***P < 0.001. (c) RNA-seq analysis of SOX2 and IGF2 expression in ESC (HUES3) and differentiated fibroblast cells from GSE73211 database. (d) ChIP-qPCR analysis to assess H3K4me3 levels of IGF2 region in SOX2-expressing T24 cell (left) and SOX2-silenced 5637 cells (right). **P < 0.01, ***P < 0.001. Primers targeting the H3K4me3 signal of IGF2 were designed at chr11: 2,158,962–2,159,038. (e) qPCR and immunoblotting analysis of IGFBP1 expression in SOX2-overexpressing T24 cells (left) and SOX2-silenced 5637 cells (right). Results are the average of three replicates and expressed as the mean ± S.D. *P < 0.05, ***P < 0.001.